Harnessing the Biomimetic Effect of Macromolecular Crowding in the Cell-Derived Model of Clubfoot Fibrosis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F24%3A00599455" target="_blank" >RIV/67985823:_____/24:00599455 - isvavai.cz</a>
Alternative codes found
RIV/44555601:13450/24:43898502 RIV/00216208:11110/24:10483788 RIV/00216208:11130/24:10483788 RIV/00216208:11310/24:10483788 RIV/00064211:_____/24:W0000009
Result on the web
<a href="https://doi.org/10.1021/acs.biomac.4c00653" target="_blank" >https://doi.org/10.1021/acs.biomac.4c00653</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.biomac.4c00653" target="_blank" >10.1021/acs.biomac.4c00653</a>
Alternative languages
Result language
angličtina
Original language name
Harnessing the Biomimetic Effect of Macromolecular Crowding in the Cell-Derived Model of Clubfoot Fibrosis
Original language description
Fibrotic changes in pediatric clubfoot provide an opportunity to improve corrective therapy and prevent relapses with targeted drugs. This study defines the parameters of clubfoot fibrosis and presents a unique analysis of a simple pseudo-3D in vitro model for disease-specific high-throughput drug screening experiments. The model combines clubfoot-derived fibroblasts with a biomimetic cultivation environment induced by the water-soluble polymers Ficoll and Polyvinylpyrrolidone, utilizing the principle of macromolecular crowding. We achieved higher conversion of soluble collagen into insoluble collagen, accelerated formation of the extracellular matrix layer and upregulated fibrosis-related genes in the mixed Ficoll environment. To test the model, we evaluated the effect of a potential antifibrotic drug, minoxidil, emphasizing collagen content and cross-linking. While the model amplified overall collagen deposition, minoxidil effectively blocked the expression of lysyl hydroxylases, which are responsible for the increased occurrence of specific collagen cross-linking in various fibrotic tissues. This limited the formation of collagen cross-link in both the model and control environments. Our findings provide a tool for expanding preclinical research for clubfoot and similar fibroproliferative conditions.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30211 - Orthopaedics
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biomacromolecules
ISSN
1525-7797
e-ISSN
1526-4602
Volume of the periodical
25
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
18
Pages from-to
6485-6502
UT code for WoS article
001302826500001
EID of the result in the Scopus database
2-s2.0-85202902215