Chiral supercritical fluid chromatography-mass spectrometry with liquid chromatography fractionation for the characterization of enantiomeric composition of fatty acid esters of hydroxy fatty acids
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F25%3A00617569" target="_blank" >RIV/67985823:_____/25:00617569 - isvavai.cz</a>
Alternative codes found
RIV/62690094:18470/25:50022265
Result on the web
<a href="https://doi.org/10.1016/j.aca.2025.343735" target="_blank" >https://doi.org/10.1016/j.aca.2025.343735</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2025.343735" target="_blank" >10.1016/j.aca.2025.343735</a>
Alternative languages
Result language
angličtina
Original language name
Chiral supercritical fluid chromatography-mass spectrometry with liquid chromatography fractionation for the characterization of enantiomeric composition of fatty acid esters of hydroxy fatty acids
Original language description
Background:Fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of endogenous bioactive lipids with promising therapeutic potential for diabetes and inflammation. They represent complex mixtures of different isomers whose biological functions are the subject of investigation. Highly selective methods are required to characterize the composition of enantiomers in biological samples composed of many isobars and regioisomers. We aimed to develop a method for characterizing the enantiomeric composition of FAHFAs in biological samples using supercritical fluid chromatography-mass spectrometry (SFC-MS).Results:The influence of key chromatographic parameters, such as column chemistry, mobile phase composition, and gradient, on the separation efficiency of 21 commercially available FAHFA regioisomers without stated absolute configuration and 4 FAHFA enantiomers was assessed. The optimized SFC-MS method utilizes a chiral column based on a tris-(3-chloro-5-methylphenylcarbamate) derivative of amylose (Lux i-Amylose-3) and acetonitrile-methanol mobile phase modifier, enabling fast enantioseparation of most FAHFA racemic pairs in 5 min. However, the SFC separation of FAHFA regioisomers was less effective, limiting its applicability to complex biological samples. To address this, we propose an offline two-dimensional separation approach with reversed-phase liquid chromatography for isolating FAHFA regioisomers, followed by chiral SFC-MS analysis of fractions. The suitability of the method was demonstrated by characterizing the enantiomeric composition of FAHFA in white adipose tissue and rice samples. The chiral analysis revealed the presence of both R- and S-FAHFA isomers in the samples, with one enantiomer being predominant.Significance:The developed approach represents a proof of concept for the use of SFC-MS with LC prefractionation for the characterization of FAHFA enantiomeric composition in complex biological samples, providing a valuable tool for future research on the biological roles of bioactive lipids in health and disease.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytica Chimica Acta
ISSN
0003-2670
e-ISSN
1873-4324
Volume of the periodical
1345
Issue of the periodical within the volume
1 April
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
7
Pages from-to
343735
UT code for WoS article
001417266400001
EID of the result in the Scopus database
2-s2.0-85216500364