Identification of two metallothioneins in Agaricus crocodilinus reveals gene duplication and domain expansion, a pattern conserved across fungal species
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985831%3A_____%2F25%3A00637508" target="_blank" >RIV/67985831:_____/25:00637508 - isvavai.cz</a>
Alternative codes found
RIV/61389005:_____/25:00637663 RIV/60461373:22330/25:43932233 RIV/60461373:22340/25:43932233
Result on the web
<a href="https://link.springer.com/article/10.1007/s10534-025-00721-6" target="_blank" >https://link.springer.com/article/10.1007/s10534-025-00721-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10534-025-00721-6" target="_blank" >10.1007/s10534-025-00721-6</a>
Alternative languages
Result language
angličtina
Original language name
Identification of two metallothioneins in Agaricus crocodilinus reveals gene duplication and domain expansion, a pattern conserved across fungal species
Original language description
Agaricus crocodilinus (Agaricaceae), an edible saprotrophic mushroom, accumulates high concentrations of cadmium (Cd) in unpolluted environments. This study investigates whether this species has evolved mechanisms to store Cd complexed with metallothioneins (MTs), proteins that bind heavy metal ions via cysteinyl (Cys)–thiolate bonds, how these MTs originated, and how similar mechanisms are present in other fungal species. Size exclusion chromatography revealed that a substantial fraction of Cd in A. crocodilinus sporocarps was sequestered in a 3.4 kDa complex containing Cys-rich peptides. Screening a sporocarp cDNA expression library in a Cd-sensitive Saccharomyces cerevisiae strain identified two MT transcripts, AcMT1 and AcMT2, encoding 49-amino acid (AA) AcMT1 with 10 Cys and 32-AA AcMT2 with 7 Cys. The presence of AcMT2 in the 3.4 kDa Cd–peptide complex isolated from sporocarp was confirmed by mass spectrometry. In mycelial isolates exposed to heavy metals, AcMT1 was more strongly upregulated, while AcMT2 was more expressed under normal conditions. Sequence comparisons revealed that AcMT2 is closer to the ancestral gene, whereas AcMT1 is a more recent duplicate. Combined bioinformatic and functional evidence supports AcMT2 as a constitutively expressed MT involved in Cd binding in the sporocarp, while AcMT1, though more inducible in mycelia and more protective in yeast, appears to serve a transient detoxification role. Moreover, the gene duplication and domain rearrangement mechanism underlying this MT diversification was also identified in other Agaricales and Boletales species.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10612 - Mycology
Result continuities
Project
<a href="/en/project/GA19-06759S" target="_blank" >GA19-06759S: Cadmium hyperaccumulation in macrofungi: from isotopes to proteins and bacterial communities</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biometals
ISSN
0966-0844
e-ISSN
1572-8773
Volume of the periodical
38
Issue of the periodical within the volume
5
Country of publishing house
DE - GERMANY
Number of pages
17
Pages from-to
1569-1585
UT code for WoS article
001531121400001
EID of the result in the Scopus database
2-s2.0-105010946311