HPLC-MS Methodology for R. carthamoides Extract Quality Evaluation: A Simultaneous Determination of Eight Bioactive Compounds
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985858%3A_____%2F22%3A00563744" target="_blank" >RIV/67985858:_____/22:00563744 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/22:43925288 RIV/60461373:22340/22:43925288
Result on the web
<a href="https://www.mdpi.com/1424-2818/14/10/880" target="_blank" >https://www.mdpi.com/1424-2818/14/10/880</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/d14100880" target="_blank" >10.3390/d14100880</a>
Alternative languages
Result language
angličtina
Original language name
HPLC-MS Methodology for R. carthamoides Extract Quality Evaluation: A Simultaneous Determination of Eight Bioactive Compounds
Original language description
Classified as an adaptogen, Maral root (Rhaponticum carthamoides, Leuzea carthamoides) is a herb that has long been used in Siberian as well as Russian alternative medicine. With over 200 substances found, this plant is a great source of bioactive compounds which have significant beneficial effects on human health and physical enhancement. Simultaneous quantification of the eight most therapeutic and abundant substances, i.e., 20-hydroxyecdysone (20-HE), kaempferol, hesperetin, quercetin, chlorogenic acid, N-feruloyl serotonin, cynaropicrin, and tracheloside belonging to various groups, such as ecdysteroids, flavonoids, phenolics, sesquiterpenes, and lignans, was performed for the first time through validated HPLC-MS. The evaluated parameters for method validation showed excellent linearity with R-2 higher than 0.996, stability under various environmental factors with % RSD <= 2%, and recovery between 97 and 103% for all the studied compounds. Other validation parameters including selectivity, sensitivity, and precision were found to be within the acceptance criteria. The results of the stability studies provide information on the best combination of conditions for sample handling and storage. Generally, for almost every compound, exposure to light and elevated temperature for 96 h led to degradation, nevertheless, the acidic environment was beneficial for most of them.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20401 - Chemical engineering (plants, products)
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Diversity
ISSN
1424-2818
e-ISSN
1424-2818
Volume of the periodical
14
Issue of the periodical within the volume
10
Country of publishing house
CH - SWITZERLAND
Number of pages
10
Pages from-to
880
UT code for WoS article
000872644700001
EID of the result in the Scopus database
2-s2.0-85140823750