Determination of Selected Biologically Active Substances in Dietary Supplements and the Adaptogenic Herb Rhaponticum carthamoides Using HPLC-MS/MS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F25%3A43931534" target="_blank" >RIV/60461373:22330/25:43931534 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007/s12161-025-02805-y" target="_blank" >https://link.springer.com/article/10.1007/s12161-025-02805-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12161-025-02805-y" target="_blank" >10.1007/s12161-025-02805-y</a>
Alternative languages
Result language
angličtina
Original language name
Determination of Selected Biologically Active Substances in Dietary Supplements and the Adaptogenic Herb Rhaponticum carthamoides Using HPLC-MS/MS
Original language description
Rhaponticum carthamoides (Willd.) Iljin, widely known as maral root, is an adaptogenic herb that has been used in traditional medicine for centuries. Several different classes of compounds were identified in R. carthamoides, including phytosteroids, flavonoids, and phenolic acids. Nowadays, R. carthamoides is available on the market in many forms, such as dried root, milled plant, capsules, and tinctures. This study presents the development and optimisation of four HPLC-MS/MS methods for the determination of 37 selected bioactive compounds in both plant and dietary supplements. Due to insufficient ionisation at the ion source, stigmasterol and ss-sitosterol required derivatization, which was developed and optimised using 2-fluoro-1-methylpyridinium p-toluenesulfonate. The dried plant samples were extracted with 60% ethanol or tert-butyl methyl ether. The results showed that chlorogenic acid and 20-hydroxyecdysone were the most abundant. To reduce the dimensionality of the obtained data, factor analysis was applied. Based on the distribution of selected compounds, four clusters were observed: tinctures, seeds, root, and shoot systems of the herb. Using the developed methods, selected bioactive compounds in the herb and dietary supplements were successfully quantified, and their distribution in different organs of R. carthamoides was clarified.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40500 - Other agricultural sciences
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Food Analytical Methods
ISSN
1936-9751
e-ISSN
1936-976X
Volume of the periodical
Neuveden
Issue of the periodical within the volume
05.05.2025
Country of publishing house
US - UNITED STATES
Number of pages
18
Pages from-to
nestránkováno
UT code for WoS article
001480971800001
EID of the result in the Scopus database
2-s2.0-105004035105