Advanced Microfluidic Platform for Tumor Spheroid Formation and Cultivation Fabricated from OSTE+ Polymer

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985858%3A_____%2F24%3A00599469" target="_blank" >RIV/67985858:_____/24:00599469 - isvavai.cz</a>

Alternative codes found

RIV/44555601:13440/24:43898493

Result on the web

<a href="https://link.springer.com/article/10.1007/s13206-024-00167-x" target="_blank" >https://link.springer.com/article/10.1007/s13206-024-00167-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13206-024-00167-x" target="_blank" >10.1007/s13206-024-00167-x</a>

Result language

angličtina

Original language name

Advanced Microfluidic Platform for Tumor Spheroid Formation and Cultivation Fabricated from OSTE+ Polymer

Original language description

In the evolving landscape of cancer research, 3D cell cultures, particularly tumor cell spheroids, are increasingly preferred in drug screening due to their enhanced mimicry of in vivo tumor environments, especially in drug resistance aspects. However, the consistent formation of uniform spheroids and their precise manipulation remain complex challenges. Among various methodologies, droplet microfluidics emerges as a highly effective approach for tumor spheroid formation. This paper introduces a novel, multifaceted microfluidic system that streamlines the entire spheroid cultivation process: generating tumor spheroids from cell suspensions within individual droplets, merging these droplets into a continuous aqueous phase once spheroid formation is complete, and transferring the spheroids to a specialized cultivation area within the chip, equipped with trapping elements for extended cultivation in perfusion mode. Remarkably, this process requires no hydrogel encapsulation or external handling, as all operations are conducted within the microfluidic chip. Fabricated from the innovative OSTE+ (off-stoichiometry thiol-ene epoxy) polymer, the chip is designed for repeated use. To show its efficacy, we successfully formed spheroids from MCF-7, GAMG, and U87 cell lines in our system and compared them with spheroids prepared by a traditional agarose microwell method. Additionally, our methodology has successfully enabled the in-chip release of spheroids from droplets, followed by their effective trapping for subsequent cultivation, a process we have exemplified with MCF-7 spheroids. To our knowledge, this research represents the first instance of a fully integrated droplet microfluidic platform achieving scaffoldless tumor spheroid formation and handling. Our method holds promise for improving high-throughput, automated procedures in the formation, transfer, and cultivation of tumor cell spheroids.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)

Project

<a href="/en/project/LM2023066" target="_blank" >LM2023066: Nanomaterials and Nanotechnologies for Environment Protection and Sustainable Future</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BioChip Journal

ISSN

1976-0280

e-ISSN

2092-7843

Volume of the periodical

18

Issue of the periodical within the volume

3

Country of publishing house

KR - KOREA, REPUBLIC OF

Number of pages

17

Pages from-to

393-409

UT code for WoS article

001280665000001

EID of the result in the Scopus database

2-s2.0-85200037919