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Comparison of E-coli O157 : H7 preparation methods used for detection with surface plasmon resonance sensor

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F05%3A00023066" target="_blank" >RIV/67985882:_____/05:00023066 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Comparison of E-coli O157 : H7 preparation methods used for detection with surface plasmon resonance sensor

  • Original language description

    This paper reports the detection of Escherichia coli (E. coli) O157:H7 with a surface plasmon resonance (SPR) sensor using three sample preparation methods: untreated (viable), heat-killed then soaked in 70% ethanol, and detergent-lysed. The limit of detection (LOD) for each method was established. Detergent-lysed samples produce the lowest LOD at 10E4 cfu/ml, while the LOD is 10E5 cfu/ml for heat-killed samples and 10E6 cfu/ml for untreated samples, respectively.

  • Czech name

    Srovnání metod přípravy E-coli O157 : H7 pro využití v senzorech s povrchovými plasmony

  • Czech description

    Tato práce se zabývá detekcí Escherichia coli (E. coli) O157:H7 pomocí senzoru s povrchovými plasmony pro E. coli připravené třemi různými způsoby: bez úpravy, tepelně upravené a loužené v 70% etanolu a upravené pomocí detergentu. Pro každou metodu byl určen detekční limit. E. coli upravená v detergentu byla detekovatelná v koncentracích 10E4 cfu/ml.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    JB - Sensors, detecting elements, measurement and regulation

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2005

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Sensors and Actuators. B - Chemical and Biochemical Sensors

  • ISSN

    0925-4005

  • e-ISSN

  • Volume of the periodical

    107

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    7

  • Pages from-to

    202-208

  • UT code for WoS article

  • EID of the result in the Scopus database