Label-Free Biosensing in Complex Media: A Referencing Approach

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F13%3A00396516" target="_blank" >RIV/67985882:_____/13:00396516 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1021/ac401062m" target="_blank" >http://dx.doi.org/10.1021/ac401062m</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/ac401062m" target="_blank" >10.1021/ac401062m</a>

Result language

angličtina

Original language name

Label-Free Biosensing in Complex Media: A Referencing Approach

Original language description

We present a novel approach to reference-compensated label-free affinity biosensing in complex media. Unlike conventional approaches that employ surfaces with different biological functionalities in the detection and reference channels to produce a reference-compensated sensor response, the new approach (referred as to single surface referencing (SSR)) uses a single functionalized surface split into the detection and reference channel to which complex sample (detection channel) and complex sample mixedwith biomolecules binding to the analyte and thus inhibiting the binding of the analyte to the functionalized surface (reference channel) is introduced. This approach ensures that (i) only the detection channel captures the analyte and (ii) nonspecific binding incurred in the detection and reference channels are the same. We evaluate this approach in a model biosensing experiment, detection of a cancer biomarker carcinoembryonic antigen (CEA) in blood plasma using antibody against CEA an

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

JB - Sensors, detecting elements, measurement and regulation

OECD FORD branch

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Project

<a href="/en/project/LH11102" target="_blank" >LH11102: Surface Plasmon Resonance Based Biosensing Using Hybrid Nanoplasmonic Materials Fabricated by Self-Assembly Processes</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical Chemistry

ISSN

0003-2700

e-ISSN

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Volume of the periodical

85

Issue of the periodical within the volume

12

Country of publishing house

US - UNITED STATES

Number of pages

4

Pages from-to

5637-5640

UT code for WoS article

000320749200005

EID of the result in the Scopus database

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