Monitoring RAYT activity by surface plasmon resonance biosensor
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F15%3A00452057" target="_blank" >RIV/67985882:_____/15:00452057 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/15:00452057
Result on the web
<a href="http://dx.doi.org/10.1007/s00216-015-8491-y" target="_blank" >http://dx.doi.org/10.1007/s00216-015-8491-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00216-015-8491-y" target="_blank" >10.1007/s00216-015-8491-y</a>
Alternative languages
Result language
angličtina
Original language name
Monitoring RAYT activity by surface plasmon resonance biosensor
Original language description
The process of DNA transposition involves the binding, cleavage, and recombination of specific DNA segments (transposable elements, TE) and is catalyzed by special enzymes encoded by the TE transposases. REP-associated tyrosine transposases (RAYTs) are aclass of Y1 nucleases related to the IS200/IS605 transposases associated with a bacterial TE known as repetitive extragenic palindrome elements (REPs). Although RAYT has been subject of numerous studies, where DNA binding and cleavage by RAYT have beenconfirmed for Escherichia coli, the molecular mechanism of DNA insertion has not been fully understood. In this work, it is demonstrated that surface plasmon resonance (SPR) biosensor technology combined with a system of DNA hairpin probes (mimicking thenatural REP sequence) and short oligonucleotides (ONs) can provide a rapid and real-time platform for monitoring and quantification of RAYT activity. We utilized RAYT from E. coli (strain MG1655) as a model system, where we evaluated its
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
JA - Electronics and optoelectronics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP305%2F12%2F1801" target="_blank" >GAP305/12/1801: Molecular mechanisms of association of a novel type of transposase with repetitive palindromic elements</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical and Bioanalytical Chemistry
ISSN
1618-2642
e-ISSN
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Volume of the periodical
407
Issue of the periodical within the volume
14
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
3985-3993
UT code for WoS article
000354190300009
EID of the result in the Scopus database
2-s2.0-84939548760