Tubulin response to intense nanosecond-scale electric field in molecular dynamics simulation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F19%3A00518300" target="_blank" >RIV/67985882:_____/19:00518300 - isvavai.cz</a>

Result on the web

<a href="https://www.nature.com/articles/s41598-019-46636-4.pdf?origin=ppub" target="_blank" >https://www.nature.com/articles/s41598-019-46636-4.pdf?origin=ppub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-019-46636-4" target="_blank" >10.1038/s41598-019-46636-4</a>

Result language

angličtina

Original language name

Tubulin response to intense nanosecond-scale electric field in molecular dynamics simulation

Original language description

Intense pulsed electric fields are known to act at the cell membrane level and are already being exploited in biomedical and biotechnological applications. However, it is not clear if electric pulses within biomedically-attainable parameters could directly influence intra-cellular components such as cytoskeletal proteins. If so, a molecular mechanism of action could be uncovered for therapeutic applications of such electric fields. To help clarify this question, we first identified that a tubulin heterodimer is a natural biological target for intense electric fields due to its exceptional electric properties and crucial roles played in cell division. Using molecular dynamics simulations, we then demonstrated that an intense - yet experimentally attainable - electric field of nanosecond duration can affect the b beta-tubulin's C-terminus conformations and also influence local electrostatic properties at the GTPase as well as the binding sites of major tubulin drugs site. Our results suggest that intense nanosecond electric pulses could be used for physical modulation of microtubule dynamics. Since a nanosecond pulsed electric field can penetrate the tissues and cellular membranes due to its broadband spectrum, our results are also potentially significant for the development of new therapeutic protocols

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20201 - Electrical and electronic engineering

Project

<a href="/en/project/GA17-11898S" target="_blank" >GA17-11898S: Nanosecond electric pulses for modulation of microtubule dynamics</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

10477

UT code for WoS article

000476468700005

EID of the result in the Scopus database

2-s2.0-85069477341