Microfluidic on-chip microwave sensing of the self-assembly state of tubulin

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F21%3A00543281" target="_blank" >RIV/67985882:_____/21:00543281 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.snb.2020.129068" target="_blank" >https://doi.org/10.1016/j.snb.2020.129068</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.snb.2020.129068" target="_blank" >10.1016/j.snb.2020.129068</a>

Result language
angličtina
Original language name
Microfluidic on-chip microwave sensing of the self-assembly state of tubulin
Original language description
Self-assembly is at the heart of many promising nanoscience technologies as well as at the core of life processes. Tubulin proteins self-assemble into microtubules, tube-like structures that are essential in cellular functions such as cell division and intracellular transport and also a major target in cancer therapies. Therefore, it is crucial to develop efficient methods for monitoring tubulin self-assembly at the molecular level. To mitigate the limitations of current methods, we propose a new approach based on a microwave lab-on-a-chip method to monitor tubulin self-assembly states. To that end, we designed a dedicated microwave platform with integrated microfluidics with a sensing volume of < 30 nL. In parallel, we used a standard bulk light-scattering-based method to assess the tubulin self-assembly and to validate the microwave chip results. Using our chip, for the first time, we demonstrate that the self-assembly state of tubulin into microtubules can be monitored using microwave microfluidics technology. Our results introduce a novel label-free electromagnetic monitoring and analytical method for bionanotechnology and biomedicine applications that can potentially be integrated into advanced microscopy systems
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
20201 - Electrical and electronic engineering

Project
<a href="/en/project/GX20-06873X" target="_blank" >GX20-06873X: SubTHz on-chip devices for controlling protein nanomachines</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)