Occurrence of restriction?modification systems in ruminal butyrate-producing bacteria
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F05%3A00027362" target="_blank" >RIV/67985904:_____/05:00027362 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Occurrence of restriction?modification systems in ruminal butyrate-producing bacteria
Original language description
Thirty-five strains of ruminal bacteria belonging to the former Butyrivibrio fibrosolvens species were screened for the presence of site-specific restriction endonuclease and modification methyltransferase activities. The recognition sequences and otimalreaction conditions for seven of them were determined. Five enzymes were found to be isoschizomers of type II endonucleases, one was type IIS (FokI) and two remained unknown. The otimal reaction buffer was found to be a low ionic strength buffer and allenzymes possessed sufficient activity at 39°C. The presence of DNA modification among all strains was also determined.
Czech name
Výskyt restrikčně-modifikačního systému u bachorových bakterií produkujících kyselinu máselnou
Czech description
Byl popsán restrikčně modifikační systém u anaerobních bakterií rodů Butyrivibrio a Pseudobutyrivibrio. Většinou se jednalo o endonukleasy II typu s aktivitou EcoRV, NsiI, AseI, a SauI. Byla zjištěna i endonukleasa IIS typu. Dvě aktivity zatím nejsou ještě popsány. Všechny izoláty se specifickými restriktasami obsahovaly i DNA modifikační metyltransferasy.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/IBS5045112" target="_blank" >IBS5045112: Transformation system for rumen anaerobic bacterium Butyrivibrio fibrisolvens</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Anaerobe
ISSN
1075-9964
e-ISSN
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Volume of the periodical
11
Issue of the periodical within the volume
-
Country of publishing house
GB - UNITED KINGDOM
Number of pages
5
Pages from-to
280-284
UT code for WoS article
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EID of the result in the Scopus database
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