Effects of selected endocrine disruptors on meiotic maturation, cumulus expansion, syntesis of hyaluronan and progesterone by porcine oocyte-cumulus complexes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F09%3A00323300" target="_blank" >RIV/67985904:_____/09:00323300 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Effects of selected endocrine disruptors on meiotic maturation, cumulus expansion, syntesis of hyaluronan and progesterone by porcine oocyte-cumulus complexes

Original language description

Before ovulation, cumulus cells synthesize a large amount of hyaluronan (HA) that is organized in an extracellular matrix.This process is called cumulus expansion. We assessed effects of endocrine disruptors (bisphenol A, BPA, 4-chloro-3-methyl phenol, CMP, di(2-ethylhexyl) phthalate, DEHP, and benzyl butyl phthalate, BBP) on FSH-induced meiotic maturation of porcine oocyte-cumulus complexes (OCC) cultured in vitro. BPA and CMP, significantly affected meiotic maturation of OCC. The number of oocytes that underwent GVBD; 78.7% and 72.4% as well as the rate of oocytes that reached M II; 50% and 53.6%, were decreased compared to control: GVBD-89.6% ; M II- 81.5%. FSH-stimulated expansion of cumulus was altered by the highest concentration of BPA and CMP (70% and 64% vs. 80.3% in control). Although BPA did not alter FSH-stimulated HA synthesis by cumulus cells, its incorporation within the complex was reduced to a half of control value. Progesterone production by OCC was significantly chan

Czech name

Vliv vybraných endokrinních disruptorů na meiotické zrání, expanzi kumulu, syntézu hyaluronové kyseliny a progesteronu oocyt-kumulárními komplexy prasat.

Czech description

Kumulární buňky, před ovulací, syntetizují hyaluronovou kyselinu (HA), která se podílí jak na organizaci mezibuněčné hmoty ("expanze kumulu") tak i na oplození. Sledovali jsme vliv endokrinních disruptorů (bisphenol A, BPA; 4-chloro-3-methyl phenol, CMP;di(2-ethylhexyl) phthalate, DEHP; a benzyl butyl phthalate, BBP) na FSH-stimulované meiotické zrání a expanzi oocyt-kumulárních komplexů (OCC) prasat v průběhu in vitro kultivace. BPA i CMP, významně ovlivnily meiotické zrání oocytů. Počet oocytů, u kterých došlo k rozpadu zárodečného váčku (GVBD; 78,7% a 72,4%) jako i počet oocytů, které se vyvíjely do metafáze II (M II; 50% a 53,6%), byl snížen v porovnaní s kontrolou ( GVBD- 89,6%; M II- 81,5%). FSH-stimulovaná expanze kumulu byla snížená nejvyšší koncentrací BPA a CMP (70% a 64% vs 80,3% kontrola). Ačkoliv BPA nezměnil FSH indukovanou syntézu HA kumulárními buňkami, její zabudováni do komplexu bylo redukováno na polovinu kontrolní hodnoty. Produkce progesteronu OCC byla významně zm

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA305%2F05%2F0960" target="_blank" >GA305/05/0960: Immunodetection of structural constituents in cumulus -extracellular matrix, that are crucial for female fertility.</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Toxicology in Vitro

ISSN

0887-2333

e-ISSN

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Volume of the periodical

23

Issue of the periodical within the volume

-

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

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UT code for WoS article

000264510200002

EID of the result in the Scopus database

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