Caspase Inhibition Affects the Expression of Autophagy-Related Molecules in Chondrocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F21%3A00553617" target="_blank" >RIV/67985904:_____/21:00553617 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16170/21:43878541

Result on the web

<a href="https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=14298856883" target="_blank" >https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=14298856883</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1177/1947603520938444" target="_blank" >10.1177/1947603520938444</a>

Result language

angličtina

Original language name

Caspase Inhibition Affects the Expression of Autophagy-Related Molecules in Chondrocytes

Original language description

Objective. Caspases, cysteine proteases traditionally associated with apoptosis and inflammation, have recently been identified as important regulators of autophagy and reported within the growth plate, a cartilaginous part of the developing bone. The aim of this research was to identify novel autophagy-related molecules affected by inhibition of pro-apoptotic caspases in chondrocytes.Design. Chondrocyte micromasses derived from mouse limb buds were treated with pharmacological inhibitors of caspases. Autophagy-related gene expression was examined and possible novel molecules were confirmed by real-time polymerase chain reaction and immunocytofluorescence. Individual caspases inhibitors were used to identify the effect of specific caspases.Results. Chondrogenesis accompanied by caspase activation and autophagy progression was confirmed in micromass cultures. Expression of several autophagy-associated genes was significantly altered in the caspases inhibitors treated groups with the most prominent decrease for Pik3cg and increase of Tnfsf10. The results showed the specific pro-apoptotic caspases that play a role in these effects. Importantly, use of caspase inhibitors mimicked changes triggered by an autophagy stimulator, rapamycin, linking loss of caspase activity to an increase in autophagy.Conclusion. Caspase inhibition significantly affects regulation of autophagy-related genes in chondrocytes cultures. Detected markers are of importance in diagnostics and thus the data presented here open new perspectives in the field of cartilage development and degradation.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cartilage

ISSN

1947-6035

e-ISSN

1947-6043

Volume of the periodical

13

Issue of the periodical within the volume

2 Suppl

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

"956S"-"968S"

UT code for WoS article

000545369100001

EID of the result in the Scopus database

2-s2.0-85087462404