Cytosine methylation of plastid genome in higher plants. Fact or artefact?

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F01%3A17013016" target="_blank" >RIV/68081707:_____/01:17013016 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Cytosine methylation of plastid genome in higher plants. Fact or artefact?

Original language description

The presence of methylcytosine residues in chloroplast genome has been investigated by restriction enzyme analysis and bisulfite genomic sequencing. We found that only CCWGG sites were partially refractory to the cleavage with EcoRII enzyme. However, bisulfite genomic sequencing failed to show the presence of modified cytosine along 3 region of the rbcL gene. It is likely that the EcoRII refractivity of DNA was caused by specific cleavage properties of this enzyme rather than by methylation of its target site. In contrast to nuclear genome chloroplast genome does not seem to contain significant amounts of methylcytosine residues

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2001

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Science

ISSN

0168-9452

e-ISSN

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Volume of the periodical

160

Issue of the periodical within the volume

4

Country of publishing house

IE - IRELAND

Number of pages

9

Pages from-to

585-593

UT code for WoS article

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EID of the result in the Scopus database

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