Transposition of IS10 from the host Escherichia coli genome to a plasmid may lead to cloning artefacts.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F01%3A17013025" target="_blank" >RIV/68081707:_____/01:17013025 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Transposition of IS10 from the host Escherichia coli genome to a plasmid may lead to cloning artefacts.
Original language description
The present paper describes and example of bacterial IS10 transposition from genome to a plasmid during cloning steps in a laboratory. The database search revealed unusually high frequency of IS10 elements in cloned eukaryotic sequences. It is estimatedthat approximately every thousandth clone in a database contains an IS contamination. We recommend checking submitted sequences for the presence of IS10 and other IS elements. In addition, DNA databases should be corrected by removing contaminating IS sequences
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Genetics and Genomics
ISSN
1617-4615
e-ISSN
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Volume of the periodical
266
Issue of the periodical within the volume
2
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
216-222
UT code for WoS article
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EID of the result in the Scopus database
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