Histone H4 acetylation patterns during seed germination and early plant development.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F03%3A17033077" target="_blank" >RIV/68081707:_____/03:17033077 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Histone H4 acetylation patterns during seed germination and early plant development.

Original language description

Here we studied whether early development of Silene latifolia is accompanied with changes in acetylation of nucleosomal histones H4. Using acid-urea-triton polyacrylamide gel and immunoblotting with specific antisera the histone acetylation in relationship with transcriptional activity, measured by [C-14]-uridine incorporation, was analysed in dry and germinating seeds, seedlings, and adult leaves. We show that quiescent and germinating seeds, until the root tip is released from testa, are characterisedby an absence of transcriptional activity and by a low H4 acetylation level: only mono-acetylated isoforms were present. During the postgermination period and early plantlet development, a high increase of transcriptional activity started and a sharp H4hyperacetylation, up to the penta-acetylated isoform, was detected. We conclude that epigenetic modification by nucleosomal histone deacetylation plays a role in maintenance of global genome silencing in quiescent seeds.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/IAA5004901" target="_blank" >IAA5004901: Nuclear Structure and Histone Acetylation in Plant Cells</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biologia Plantarum

ISSN

0006-3134

e-ISSN

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Volume of the periodical

46

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

23-28

UT code for WoS article

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EID of the result in the Scopus database

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