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Conformation of DNA modified by monofunctional Ru(II) arene complexes: Recognition by DNA binding proteins and repair. Relationship to cytotoxicity

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F05%3A00001319" target="_blank" >RIV/68081707:_____/05:00001319 - isvavai.cz</a>

  • Alternative codes found

    RIV/68081707:_____/06:00079041 RIV/68081707:_____/06:00359094

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Conformation of DNA modified by monofunctional Ru(II) arene complexes: Recognition by DNA binding proteins and repair. Relationship to cytotoxicity

  • Original language description

    We analyzed DNA duplexes modified at central guanine residues by monofunctional Ru(II) arene complexes [(eta(6)-arene)Ru(II)(en)(Cl)](+) (arene = tetrahydroanthracene or p-cymene, Ru-THA or Ru-CYM, respectively). These two complexes were chosen as representatives of two different classes of Ru(II) arene compounds for which initial studies revealed different binding modes: one that may involve DNA intercalation (tricyclic-ring Ru-THA) and the other (mono-ring Ru-CYM) that may not. Ru-THA is similar to20times more toxic to cancer cells than Ru-CYM. The adducts of Ru-THA and Ru-CYM have contrasting effects on the conformation, thermodynamic stability, and polymerization of DNA in vitro. In addition, the adducts of Ru-CYM are removed from DNA more efficiently than those of Ru-THA. Interestingly, the mammalian nucleotide excision repair system has low efficiency for excision of ruthenium adducts compared to cisplatin intrastrand crosslinks.

  • Czech name

    Konformace DNA modifikované monofunkčními arenovými komplexy Ru(II): rozlišení proteiny, které se váží k DNA a oprava. Vztah k cytotoxicitě

  • Czech description

    Byly analyzovány krátké duplexy DNA modifikované tak, že obsahovaly monofunkční adukty vytvořené na centrálních zbytcích guaninu monofunkčními arenovými komplexy Ru(II) {[(n6-arene)Ru(II)(en)(Cl)]+ (arene=tetrahydroanthracene nebo p-cymene]}. Pro tuto studii byly zvoleny komplexy, které se liší při své vazbě na DNA schopností vmezeřovat do DNA arenový ligand. Bylo zjištěno, že tyto dva typy komplexů, které se výrazně liší cytotoxickými účinky v nádorových buňkách, vytvářejí adukty na DNA, které mají výrazně odlišný vliv na konformaci, termodynamickou stabilitu, polymerizaci DNA a jsou také odlišně opravovány.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    BO - Biophysics

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2006

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Chemistry & Biology

  • ISSN

    1074-5521

  • e-ISSN

  • Volume of the periodical

    12

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    9

  • Pages from-to

    121-129

  • UT code for WoS article

  • EID of the result in the Scopus database