MK-886 enhances tumour necrosis factor-alpha-induced differentiation and apoptosis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F06%3A00040473" target="_blank" >RIV/68081707:_____/06:00040473 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
MK-886 enhances tumour necrosis factor-alpha-induced differentiation and apoptosis
Original language description
We investigated the role of the 5-lipoxygenase (5-LOX) pathway of arachidonic acid metabolism in tumour necrosis factor-alpha (TNF-alpha)-induced differentiation of human leukemic HL-60 cells using MK-886, an inhibitor of 5-LOX activating protein. MK-886augmented cell cycle arrest and differentiation induced by TNF-alpha; however, both effect were probably 5-LOX-independent, because a general LOX inhibitor, NDGA, had no effect. Apoptosis was significantly elevated after combined TNF-alpha and MK-886 treatment, which could be partially associated with changes of Mc1-1 protein expression. NF-ksíB signalling or activation of JNKs were not modulated by MK-886. Thus, in addition to apoptosis, MK-886 can enhance TNF-alpha-induced differentiation.
Czech name
MK-886 zvyšuje diferenciaci a apoptózu indukovanou TNF-alpha
Czech description
S využitím látky MK-886, inhibitoru proteinu aktivujícího 5-lipoxygenázu (5-LOX), byla studována role dráhy 5-LOX přeměny kyseliny arachidonové v diferenciaci indukované TNF-alpha u lidské buněčné leukemické linie HL-60. MK-886 posiloval zástavu buněčného cyklu a diferenciaci indukovanou TNF-alpha, ale tyto efekty jsou pravděpodobně nezávislé na inhibici 5-LOX, protože obecný inhibitor LOX, NDGA, neměl žádné účinky. Po kombinaci TNF-alpha a MK-886 byla významně potencována apoptóza, což částečně souviselo se změnami exprese proteinu Mc1-1. Signálování přes NF-ksíB nebo aktivace JNK nebyla MK-886 modulována. Tak, kromě apoptózy, MK-886 může zvyšovat i diferenciaci indukovanou TNF-alpha.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/GA524%2F03%2F0766" target="_blank" >GA524/03/0766: Modulation of proliferation, differentiation and apoptosis of hemopoietic cells - interactions of cytokines, drugs and lipid nutrition compounds</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cancer letters
ISSN
0304-3835
e-ISSN
—
Volume of the periodical
237
Issue of the periodical within the volume
2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
263-271
UT code for WoS article
—
EID of the result in the Scopus database
—