Thermodynamic Impact of Abasic Sites on Simulated Translesion DNA Synthesis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F14%3A00435430" target="_blank" >RIV/68081707:_____/14:00435430 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/chem.201402600" target="_blank" >http://dx.doi.org/10.1002/chem.201402600</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/chem.201402600" target="_blank" >10.1002/chem.201402600</a>

Result language

angličtina

Original language name

Thermodynamic Impact of Abasic Sites on Simulated Translesion DNA Synthesis

Original language description

Loss of a base in DNA and the creation of an abasic (apurinic/apyrimidinic, AP) site is a frequent lesion that may occur spontaneously, or as a consequence of the action of DNA-damaging agents. The AP lesion is mutagenic or lethal if not repaired. We report a systematic thermodynamic investigation by differential scanning calorimetry on the evolution, during primer extension, of a model AP site in chemically simulated DNA translesion synthesis. Incorporation of dAMP (deoxyadenosine monophosphate), as well as dTMP (deoxythymidine monophosphate), opposite an AP site is enthalpically unfavorable, although incorporation of dTMP is more enthalpically unfavorable than that of dAMP. This finding is in a good agreement with experimental data showing that AP sites block various DNA polymerases of eukaryotic and prokaryotic origin and that, if bypassed, dAMP is preferentially inserted, whereas insertion of dTMP is less likely. The results emphasize the importance of thermodynamic contributions t

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/GAP205%2F11%2F0856" target="_blank" >GAP205/11/0856: Thermodynamics of DNA damaged by physical and chemical agents</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemistry - A European Journal

ISSN

0947-6539

e-ISSN

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Volume of the periodical

20

Issue of the periodical within the volume

25

Country of publishing house

DE - GERMANY

Number of pages

5

Pages from-to

7566-7570

UT code for WoS article

000337637400002

EID of the result in the Scopus database

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