Probing the Thermodynamics of Incorporation of N-6-methyl-dATP Opposite an Abasic Site, dCMP, and dTMP During Simulated DNA Synthesis by Differential Scanning Calorimetry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F18%3A00501578" target="_blank" >RIV/68081707:_____/18:00501578 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/slct.201803565" target="_blank" >http://dx.doi.org/10.1002/slct.201803565</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/slct.201803565" target="_blank" >10.1002/slct.201803565</a>
Alternative languages
Result language
angličtina
Original language name
Probing the Thermodynamics of Incorporation of N-6-methyl-dATP Opposite an Abasic Site, dCMP, and dTMP During Simulated DNA Synthesis by Differential Scanning Calorimetry
Original language description
Previous reports indicated that when an abasic (apurinic/apyrimidinic, AP) site is bypassed by DNA polymerases, dATP is preferentially inserted. Here we evaluate, using differential scanning calorimetry, the thermodynamic changes associated with incorporation of N-6-methyl-dATP opposite an AP site, dCMP, and dTMP during simulated DNA polymerization. The results confirm that AP sites block DNA polymerases one nucleotide prior to the lesion. Thermodynamic data imply that the propensity of N-6-methyl-dAMP for elongation, when incorporated opposite an AP site, is higher than that of dAMP in agreement with a higher promutagenic potential of N-6-methyl-dATP if placed opposite a non-instructional DNA lesion, such as an AP site.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA17-09436S" target="_blank" >GA17-09436S: Translesion DNA synthesis across lesions induced by agents of biological significance. An insight into energetics, kinetics and mechanism</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ChemistrySelect
ISSN
2365-6549
e-ISSN
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Volume of the periodical
3
Issue of the periodical within the volume
46
Country of publishing house
DE - GERMANY
Number of pages
5
Pages from-to
13076-13080
UT code for WoS article
000453576900014
EID of the result in the Scopus database
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