Shape-selective recognition of DNA abasic sites by metallohelices: inhibition of human AP endonuclease 1

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00457638" target="_blank" >RIV/68081707:_____/15:00457638 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1093/nar/gkv438" target="_blank" >http://dx.doi.org/10.1093/nar/gkv438</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkv438" target="_blank" >10.1093/nar/gkv438</a>

Result language

angličtina

Original language name

Shape-selective recognition of DNA abasic sites by metallohelices: inhibition of human AP endonuclease 1

Original language description

Loss of a base in DNA leading to creation of an abasic (AP) site leaving a deoxyribose residue in the strand, is a frequent lesion that may occur spontaneously or under the action of various physical and chemical agents. Progress in the understanding ofthe chemistry and enzymology of abasic DNA largely relies upon the study of AP sites in synthetic duplexes. We report here on interactions of diastereomerically pure metallo-helical 'flexicate' complexes, bimetallic triple-stranded ferro-helicates [Fe-2(NN-NN)(3)](4+) incorporating the common NN-NN bis(bidentate) helicand, with short DNA duplexes containing AP sites in different sequence contexts. The results show that the flexicates bind to AP sites in DNA duplexes in a shape-selective manner. They preferentially bind to AP sites flanked by purines on both sides and their binding is enhanced when a pyrimidine is placed in opposite orientation to the lesion. Notably, the Lambda-enantiomer binds to all tested AP sites with higher affinit

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/GAP205%2F11%2F0856" target="_blank" >GAP205/11/0856: Thermodynamics of DNA damaged by physical and chemical agents</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

1362-4962

e-ISSN

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Volume of the periodical

43

Issue of the periodical within the volume

11

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

5297-5306

UT code for WoS article

000357886900011

EID of the result in the Scopus database

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