Direct chemical modification and voltammetric detection of glycans in glycoproteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F14%3A00440098" target="_blank" >RIV/68081707:_____/14:00440098 - isvavai.cz</a>

Alternative codes found

RIV/00209805:_____/14:#0000582

Result on the web

<a href="http://dx.doi.org/10.1016/j.elecom.2014.08.011" target="_blank" >http://dx.doi.org/10.1016/j.elecom.2014.08.011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.elecom.2014.08.011" target="_blank" >10.1016/j.elecom.2014.08.011</a>

Result language

angličtina

Original language name

Direct chemical modification and voltammetric detection of glycans in glycoproteins

Original language description

Most of the proteins are glycosylated and glycoproteins are involved in physiological and pathological processes. Detection of changes in protein glycosylation is important in early diagnosis of different diseases, including cancer. Among the methods used for the analysis of the carbohydrate components (glycans) of glycoproteins, electrochemical methods were little applied. We propose a modification of glycans directly in glycoproteins using six-valent osmium complexes followed by glycan voltammetric determination at carbon electrodes. The electrochemical responses of two glycoproteins ribonuclease B and avidin as compared to their nonglycosylated counterparts were recorded either directly in the reaction mixture or after a simple purification step. Hundreds of femtomoles of the glycoprotein were sufficient for the analysis. (C) 2014 Elsevier B.V. All rights reserved.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrochemistry Communications

ISSN

1388-2481

e-ISSN

—

Volume of the periodical

48

Issue of the periodical within the volume

NOV2014

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

4

Pages from-to

52-55

UT code for WoS article

000345208000014

EID of the result in the Scopus database

—