Effect of His(6)-tagging of anterior gradient 2 protein on its electro-oxidation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F14%3A00440148" target="_blank" >RIV/68081707:_____/14:00440148 - isvavai.cz</a>
Alternative codes found
RIV/00209805:_____/14:#0000567
Result on the web
<a href="http://dx.doi.org/10.1016/j.electacta.2014.10.125" target="_blank" >http://dx.doi.org/10.1016/j.electacta.2014.10.125</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.electacta.2014.10.125" target="_blank" >10.1016/j.electacta.2014.10.125</a>

Result language
angličtina
Original language name
Effect of His(6)-tagging of anterior gradient 2 protein on its electro-oxidation
Original language description
His-tagged and non-tagged forms of Anterior Gradient 2 (AGR2), oncoprotein and potential cancer biomarker, were studied for the first time using voltammetry at carbon electrodes. In addition to oxidation peak of Tyr and Trp, N-terminal His-tagged AGR2 form yielded characteristic electro-oxidation peak of histidine. Qualitatively similar results were obtained with other N-terminal His-tagged proteins, such as, glutathione-S-transferase, alpha-synuclein and cytochrome b5. Our results suggested that His-tag modification of proteins (commonly used in recombinant protein preparation) may change adsorption and orientation of the proteins at electrode surfaces. In absence of the His-tag in His-containing proteins, appearance of His peak was influenced by accessibility of His residues, and depended on the carbon electrode type. Oxidation His peak, in combination with Tyr and Trp oxidation responses may find use in label-free analysis of numerous proteins, including those important in biomedici
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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