P36 VOLTAMETRIC DETECTION OF DNA DELETION ON PENCIL ELECTRODE

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F14%3A00506725" target="_blank" >RIV/68081707:_____/14:00506725 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
P36 VOLTAMETRIC DETECTION OF DNA DELETION ON PENCIL ELECTRODE
Original language description
In this work, we present a new qualitative approach of detection of PCR products using electrochemistry on pencil electrodes. PCR products with an incorporated biotin-labeled dNTP (dCTP in this study) are detected via conjugated streptavidinealkaline phospatase. 1-Naphthyl phosphate (1-NP), which is dephosphorylated by alkaline phosphatase to release 1-naphthol, was used as a substrate in electrochemical detection of the PCR product. Voltammetric measurement on disposable pencil electrode is a very cheap and easy to use method. The system was optimized for plasmid DNA PCR product with potential to detect human genome DNA products and possible application in gene deletion monitoring.
Czech name
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Czech description
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis
ISBN
978-80-904959-2-0
ISSN
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e-ISSN
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Number of pages
2
Pages from-to
"Č. 2014 (2014)"
Publisher name
Institute of Analytical Chemistry AS CR
Place of publication
Brno
Event location
Brno
Event date
Oct 20, 2014
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000354547400074

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