Electrochemical and circular dichroism spectroscopic evidence of two types of interaction between [Ru(NH3)(6)](3+) and an elongated thrombin binding aptamer G-quadruplex

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00455887" target="_blank" >RIV/68081707:_____/15:00455887 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.electacta.2015.05.023" target="_blank" >http://dx.doi.org/10.1016/j.electacta.2015.05.023</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.electacta.2015.05.023" target="_blank" >10.1016/j.electacta.2015.05.023</a>

Result language

angličtina

Original language name

Electrochemical and circular dichroism spectroscopic evidence of two types of interaction between [Ru(NH3)(6)](3+) and an elongated thrombin binding aptamer G-quadruplex

Original language description

In the field of electrochemical biosensing based on DNA probes immobilised at electrode surfaces the [Ru(NH3)(6)](3+) redox marker is commonly used for quantitative characterisation and/or detection purposes. Although it is generally assumed that the interactions between the hexaammineruthenium(III) cation and DNA are solely of electrostatic nature, and thus non-specific in essence, it is shown herein that this is not the case for a sequence containing a G-quadruplex part. The present work investigatesthe interactions of [Ru(NH3)(6)](3+) with an oligonucleotide (21-mer) consisting of the thrombin binding aptamer sequence (a G-quadruplex) elongated by a 5'-overhang of six nucleotides. Combining circular dichroism and electrochemical analyses by differential pulse voltammetry, alternating current voltammetry and cyclic voltammetry, it is demonstrated that the overall interaction stoichiometry (number of [Ru(NH3)(6)](3+) bound to one DNA strand) is lower than the 7 [Ru(NH3)(6)](3+) per s

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrochimica acta

ISSN

0013-4686

e-ISSN

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Volume of the periodical

179

Issue of the periodical within the volume

OCT 2015

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

84-92

UT code for WoS article

000362292200012

EID of the result in the Scopus database

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