Possibility of Using Purine Oxidation Signals for DNA Sequence Analysis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00506713" target="_blank" >RIV/68081707:_____/15:00506713 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Possibility of Using Purine Oxidation Signals for DNA Sequence Analysis

Original language description

Signals provided by electrochemical oxidation of single stranding DNA on the surface of pyrolytic graphite electrode, which correspond to the oxidation of adenine and guanine (A and G) are known for over forty years. The mechanism of oxidation of free A and G as well as nucleoside mono-, and triphosphates of these bases is know but the mechanism of oxidation of purines in the DNA still remains unexplained. Ratiometry of A and G signals could be a new tool for DNA sequence analysis, if signals of A and G oxidation obtained from single-stranded DNA adsorbed at the electrode surface are proportional to the number of oxidized As and Gs in studied samples. Our preliminary results suggest that there is a correlation, yet we encountered problems, which yet has to be addressed and explained before this method could be applied for DNA analysis.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project

<a href="/en/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Center of novel approaches to bioanalysis and molecular diagnostics</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

XXXV. Moderní elektrochemické metody. Sborník přednášek mezinárodní odborné konference

ISBN

978-80-905221-3-8

ISSN

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e-ISSN

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Number of pages

3

Pages from-to

"Č. 2015 (2015)"

Publisher name

Srsenová Lenka - Best Servis

Place of publication

Ústí nad Labem

Event location

Jetřichovice

Event date

May 18, 2015

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000358792900052