Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F16%3A00472055" target="_blank" >RIV/68081707:_____/16:00472055 - isvavai.cz</a>

Alternative codes found

RIV/00159816:_____/16:00064994 RIV/00216224:14310/16:00089517

Result on the web

<a href="http://dx.doi.org/10.1063/1.4941776" target="_blank" >http://dx.doi.org/10.1063/1.4941776</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1063/1.4941776" target="_blank" >10.1063/1.4941776</a>

Result language

angličtina

Original language name

Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro

Original language description

The analysis of digital video output enables the non-invasive screening of various active biological processes. For the monitoring and computing of the beating parameters of cardiomyocytes in vitro, CB Analyser (cardiomyocyte beating analyser) software was developed. This software is based on image analysis of the video recording of beating cardiomyocytes. CB Analyser was tested using cardiomyocytes derived from mouse embryonic stem cells at different stages of cardiomyogenesis. We observed that during differentiation (from day 18), the beat peak width decreased, which corresponded to the increased speed of an individual pulse. However, the beating frequency did not change. Further, the effects of epinephrine modulating mature cardiomyocyte functions were tested to validate the CB Analyser analysis. In conclusion, data show that CB Analyser is a useful tool for evaluating the functions of both developing and mature cardiomyocytes under various conditions in vitro. (C) 2016 AIP Publishing LLC.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Review of Scientific Instruments

ISSN

0034-6748

e-ISSN

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Volume of the periodical

87

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

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UT code for WoS article

000371740900257

EID of the result in the Scopus database

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