Characterization of telomeres and telomerase from the single-celled eukaryote Giardia intestinalis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F17%3A00471934" target="_blank" >RIV/68081707:_____/17:00471934 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/17:10360731

Result on the web

<a href="http://dx.doi.org/10.1016/j.molbiopara.2016.09.003" target="_blank" >http://dx.doi.org/10.1016/j.molbiopara.2016.09.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molbiopara.2016.09.003" target="_blank" >10.1016/j.molbiopara.2016.09.003</a>

Result language

angličtina

Original language name

Characterization of telomeres and telomerase from the single-celled eukaryote Giardia intestinalis

Original language description

The ends of linear chromosomes, telomeres, are most commonly maintained by the enzyme telomerase. Our study presents the characteristics of telomeres and telomerase from the single-celled parasitic eukaryote Giardia intestinalis. Using fluorescence in situ hybridization, we localized telomeres during all stages of the trophozoite cell cycle and demonstrated differences in the observed number of telomeric foci, indicating telomere clustering. The length of Giardia telomeres was determined in different cell lines derived from WB clinical isolate using terminal restriction fragment analysis and ranged from 0.5 to 2.5 kb; moreover, a BAL-31 digestion experiment did not reveal any long interstitial telomeric sequences in the genome. Despite the absence of the specific T motif in the telomerase catalytic subunit, the presence of an active telomerase enzyme synthesising telomeric repeats in Giardia was proved by a Telomere repeat amplification protocol assay, and its localization in nuclei was determined by the expression of recombinant GiTERT. Except for the Giardia-type TAGGG telomeric repeat, Giardia telomerase was proved to synthesize in vitro also other repeat variants, TAAGG and TAAGGG. In summary, despite its unusual characteristics, including a structurally divergent but active telomerase, unique terminal sequences and relatively short telomeres, the present data support the view that the chromosomal termini in Giardia are maintained in a conservative manner that is common to other eukaryotes. (C) 2016 Elsevier B.V. All rights reserved.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/GAP305%2F12%2F1248" target="_blank" >GAP305/12/1248: Maintenance of chromosome integrity in Giardia intestinalis, a unicellular bi-nucleated eukaryote with minimalistic genome</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular and Biochemical Parasitology

ISSN

0166-6851

e-ISSN

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Volume of the periodical

211

Issue of the periodical within the volume

JAN 2017

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

31-38

UT code for WoS article

000392554100005

EID of the result in the Scopus database

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