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Protein p53 Binding to Cisplatin-modified DNA Targets Evaluated by Modification-specific Electrochemical Immunoprecipitation Assay

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F17%3A00477358" target="_blank" >RIV/68081707:_____/17:00477358 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14740/17:00100459

  • Result on the web

    <a href="http://dx.doi.org/10.1002/elan.201600480" target="_blank" >http://dx.doi.org/10.1002/elan.201600480</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/elan.201600480" target="_blank" >10.1002/elan.201600480</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Protein p53 Binding to Cisplatin-modified DNA Targets Evaluated by Modification-specific Electrochemical Immunoprecipitation Assay

  • Original language description

    Studies of protein interactions with chemically modified nucleic acids are of importance in various areas of biomolecular and biomedical research, including investigations of the binding of proteins important in medicine with DNA modified with drugs and diagnostic applications of modified DNAs in biosensing and bioanalysis. Chemical modification of DNA substrates with various species inside or outside specific protein binding sites can affect the protein-DNA recognition. In this paper we present a simple electrochemical immunoprecipitation technique designed for evaluation of the effects of antitumor drug cisplatin on the p53-DNA binding. The cisplatin-DNA adducts are utilized as electroactive labels allowing a facile determination of the p53-bound modified DNA. Effects observed using this technique accord with results of previous biochemical assays. This approach is potentially applicable in studies that deal with the influence of any electroactive DNA modifications on the protein-DNA binding.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Electroanalysis

  • ISSN

    1040-0397

  • e-ISSN

  • Volume of the periodical

    29

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    5

  • Pages from-to

    319-323

  • UT code for WoS article

    000394998000002

  • EID of the result in the Scopus database