Intrinsic Electrocatalysis in DNA

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F18%3A00506881" target="_blank" >RIV/68081707:_____/18:00506881 - isvavai.cz</a>

Alternative codes found

RIV/00209805:_____/18:00077987

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1002/celc.201700888" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1002/celc.201700888</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/celc.201700888" target="_blank" >10.1002/celc.201700888</a>

Result language

angličtina

Original language name

Intrinsic Electrocatalysis in DNA

Original language description

We show, for the first time, that DNA produces an electrocatalytic square wave voltammetric reduction peak or chronopotentiometric peak H-DNA at mercury and solid amalgam electrodes. These peaks are attributed to the catalytic hydrogen evolution reaction, allowing label-free detection of chromosomal DNA and oligodeoxynucleotides (ODNs) below parts per million and nanomolar levels, respectively. By using peak H-DNA at high current densities, native chromosomal DNA was distinguished from its denatured form. With this method, damage of DNA from human cancer cells by ionizing radiation or sonication was detected. Sensitivity of the DNA determination was greatly enhanced in the presence of [Co(NH3)(6)](3+). Chromosomal DNA and ODNs can be detected at concentrations 2-3 orders lower than any earlier voltammetric or chronopotentiometric label-free methods. In the presence of 4 mM [Co(NH3)(6)](3+), DNA from human cancer cells was easily detected at 5ng/mL, that is, below the concentration level of cell-free DNA in body fluids.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ChemElectroChem

ISSN

2196-0216

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

6

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

936-942

UT code for WoS article

000428374400014

EID of the result in the Scopus database

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