The region upstream of the telomerase reverse transcriptase gene is essential for in planta telomerase complementation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00504497" target="_blank" >RIV/68081707:_____/19:00504497 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14740/19:00107676

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0168945218314511?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0168945218314511?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plantsci.2019.01.001" target="_blank" >10.1016/j.plantsci.2019.01.001</a>

Result language

angličtina

Original language name

The region upstream of the telomerase reverse transcriptase gene is essential for in planta telomerase complementation

Original language description

Telomerase is essential for the maintenance of telomeres, structures located at the ends of linear eukaryotic chromosomes that are crucial for genomic stability. Telomerase has been frequently explored in mammals because of its activity in many types of cancers, but knowledge in plants is rather sketchy despite plants representing useful models due to peculiarities in their telomeres and telomerase biology. We studied in planta complementation of telomerase in Arabidopsis thaliana mutant plants with disrupted expression of the gene encoding the telomerase protein subunit (AtTERT) and significantly shortened telomeres. We found that the upstream region of AtTERT, previously identified as a putative minimal promoter, was essential for reconstitution of telomerase function, as demonstrated by the full or partial recovery of the telomere phenotype in mutants. In contrast, transformation by the full length AtTERT gene construct resulted in more progressive telomere shortening in mutants and even in wild type plants, despite the high level of AtTERT transcript and telomerase activity detected by in vitro assay. Thus, the telomerase protein subunit putative promoter is essential for in planta telomerase reconstitution and restoration of its catalytical activity. Contributions from other factors, including those tissue-specific, for proper telomerase function are discussed.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Science

ISSN

0168-9452

e-ISSN

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Volume of the periodical

281

Issue of the periodical within the volume

APR 2019

Country of publishing house

IE - IRELAND

Number of pages

11

Pages from-to

41-51

UT code for WoS article

000461265000005

EID of the result in the Scopus database

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