Guanine Substitutions Prevent Conformational Switch from Antiparallel to Parallel G-Quadruplex

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00511784" target="_blank" >RIV/68081707:_____/19:00511784 - isvavai.cz</a>

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/chem.201903015" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/chem.201903015</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/chem.201903015" target="_blank" >10.1002/chem.201903015</a>

Result language

angličtina

Original language name

Guanine Substitutions Prevent Conformational Switch from Antiparallel to Parallel G-Quadruplex

Original language description

Guanine quadruplexes, recently reported to form in vivo, represent a broad spectrum of non-canonical conformations of nucleic acids. The actual conformation might differ between water solutions and crowding or dehydrating solutions that better reflect the conditions in the cell. Here we show, using spectroscopic techniques, that most guanine substitutions prevent the conformational switch from antiparallel or hybrid forms to parallel ones when induced by dehydrating agents. The inhibitory effect does not depend on the position of the substitution, but, interestingly, on the type of substitution and, to some extent, on its destabilising potential. A parallel form might be induced in some cases by ligands such as N-methyl mesoporphyrin IX and even this ligand-induced switch is inhibited by guanine substitution. The ability or inability to have a conformation switch, based on actual conditions, might significantly influence potential conformation-dependent quadruplex interactions.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemistry - A European Journal

ISSN

0947-6539

e-ISSN

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Volume of the periodical

25

Issue of the periodical within the volume

58

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

13422-13428

UT code for WoS article

000487369300001

EID of the result in the Scopus database

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