Quadruplex DNA in long terminal repeats in maize LTR retrotransposons inhibits the expression of a reporter gene in yeast
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F18%3A00100852" target="_blank" >RIV/00216224:14330/18:00100852 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/18:00492512 RIV/00216305:26230/18:PU127784
Result on the web
<a href="https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-018-4563-7" target="_blank" >https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-018-4563-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12864-018-4563-7" target="_blank" >10.1186/s12864-018-4563-7</a>
Alternative languages
Result language
angličtina
Original language name
Quadruplex DNA in long terminal repeats in maize LTR retrotransposons inhibits the expression of a reporter gene in yeast
Original language description
Background Many studies have shown that guanine-rich DNA sequences form quadruplex structures (G4) in vitro but there is scarce evidence of guanine quadruplexes in vivo. The majority of potential quadruplex-forming sequences (PQS) are located in transposable elements (TEs), especially close to promoters within long terminal repeats of plant LTR retrotransposons. Results In order to test the potential effect of G4s on retrotransposon expression, we cloned the long terminal repeats of selected maize LTR retrotransposons upstream of the lacZ reporter gene and measured its transcription and translation in yeast. We found that G4s had an inhibitory effect on translation in vivo since “mutants” (where guanines were replaced by adenines in PQS) showed higher expression levels than wild-types. In parallel, we confirmed by circular dichroism measurements that the selected sequences can indeed adopt G4 conformation in vitro. Analysis of RNA-Seq of polyA RNA in maize seedlings grown in the presence of a G4-stabilizing ligand (NMM) showed both inhibitory as well as stimulatory effects on the transcription of LTR retrotransposons. Conclusions Our results demonstrate that quadruplex DNA located within long terminal repeats of LTR retrotransposons can be formed in vivo and that it plays a regulatory role in the LTR retrotransposon life-cycle, thus also affecting genome dynamics.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Result continuities
Project
<a href="/en/project/GA15-02891S" target="_blank" >GA15-02891S: Plant transposable elements and DNA conformation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BMC Genomics
ISSN
1471-2164
e-ISSN
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Volume of the periodical
19
Issue of the periodical within the volume
06 03 2018
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
184
UT code for WoS article
000427128900005
EID of the result in the Scopus database
2-s2.0-85043366153