Electrochemical Reduction and Oxidation of Six Natural 2 '-Deoxynucleosides at a Pyrolytic Graphite Electrode in the Presence or Absence of Ambient Oxygen

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00518363" target="_blank" >RIV/68081707:_____/19:00518363 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/19:00112928
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.201900417" target="_blank" >https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.201900417</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elan.201900417" target="_blank" >10.1002/elan.201900417</a>

Result language
angličtina
Original language name
Electrochemical Reduction and Oxidation of Six Natural 2 '-Deoxynucleosides at a Pyrolytic Graphite Electrode in the Presence or Absence of Ambient Oxygen
Original language description
Recently we have demonstrated that pyrolytic graphite electrodes (PGE) offer a broad analytically useful potential window, ranging from about2.0 V to +1.6 V vs. Ag|AgCl|3 M KCl, which enables to use the PGE not only for anodic measurements, but also for direct reduction of nucleobases in DNA oligonucleotides. In this follow-up study, we have focused on the electrochemical behavior of four 2 '-deoxynucleosides derived from adenine, guanine, cytosine, thymine, uracil, and 5-methyl cytosine on the PGE. On one hand we have obtained analogous primary redox responses as previously with the oligonucleotides. On the other hand, significant differences were observed, particularly when considering secondary responses involving products of the primary conversions, suggesting involvement of different mechanisms. Further we have found that presence of the ambient oxygen in the electrolyte does not dramatically affect the redox signals of the nucleosides. This finding is in contrast with DNA responses measured at the mercury-based electrodes, where deaeration prior to the measurements was necessary. We demonstrate that all studied nucleosides can be analyzed using a simple ex situ (medium exchange) procedure.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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