Serotonin and its metabolites reduce oxidative stress in murine RAW264.7 macrophages and prevent inflammation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F20%3A00524249" target="_blank" >RIV/68081707:_____/20:00524249 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/20:00117037
Result on the web
<a href="https://link.springer.com/article/10.1007/s13105-019-00714-3" target="_blank" >https://link.springer.com/article/10.1007/s13105-019-00714-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s13105-019-00714-3" target="_blank" >10.1007/s13105-019-00714-3</a>
Alternative languages
Result language
angličtina
Original language name
Serotonin and its metabolites reduce oxidative stress in murine RAW264.7 macrophages and prevent inflammation
Original language description
In this study, we focused on comparing the effects of serotonin and its metabolites on the functions of RAW264.7 cells (emphasis on oxidative burst and production of nitric oxide and cytokines), thereby expanding the scope of existing knowledge with advent of novel findings in this field. Changes in production of reactive oxygen species (ROS) by RAW264.7 cells after treatment with serotonin, N-acetylserotonin and melatonin were determined using the chemiluminescence (CL) assay. To exclude the direct scavenging effects of the studied compounds on the CL response, the antioxidant properties of all respective compounds were measured using TRAP and amperometrical method. Nitric oxide (NO) production was measured by Griess reagent and inducible NO synthase (iNOS) expression by Western blot. Cytokine production was assessed using the Mouse Cytokine Panel A Array kit and ELISA. We showed that all tested compounds were able to reduce oxidative stress, as well as inhibit production of inflammatory cytokines by macrophages. Of the tested compounds, serotonin and N-acetylserotonin were markedly better antioxidants than melatonin. In comparison, other effects of tested compounds were very similar. It can be concluded that antioxidant capacity of tested compounds is a major advantage in the early stages of inflammation. Since plasma concentrations of N-acetylserotonin and melatonin are lower than serotonin, it can be deduced that serotonin plays a key role in modulation of inflammation and the regulatory functions of immune cells, while also protecting cells against oxidative stress.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LD14030" target="_blank" >LD14030: Molecular mechanisms of serotoninergic system interactions with cells of immune system</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Physiology and Biochemistry
ISSN
1138-7548
e-ISSN
—
Volume of the periodical
76
Issue of the periodical within the volume
1
Country of publishing house
ES - SPAIN
Number of pages
12
Pages from-to
49-60
UT code for WoS article
000520009600004
EID of the result in the Scopus database
2-s2.0-85077282839