Slug-expressing mouse prostate epithelial cells have increased stem cell potential

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F20%3A00539347" target="_blank" >RIV/68081707:_____/20:00539347 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/20:00073528 RIV/00216224:14310/20:00117616 RIV/00098892:_____/20:N0000087 RIV/61989592:15110/20:73602697
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1873506120301458?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1873506120301458?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.scr.2020.101844" target="_blank" >10.1016/j.scr.2020.101844</a>

Result language
angličtina
Original language name
Slug-expressing mouse prostate epithelial cells have increased stem cell potential
Original language description
Deciphering the properties of adult stem cells is crucial for understanding of their role in healthy tissue and in cancer progression as well. Both stem cells and cancer stem cells have shown association with epithelial-to-mesenchymal transition (EMT) in various tissue types. Aiming to investigate the epithelial and mesenchymal phenotypic traits in adult mouse prostate, we sorted subpopulations of basal prostate stem cells (mPSCs) and assessed the expression levels of EMT regulators and markers with custom-designed gene expression array. The population of mPSCs defined by a Lin(-)/Sca-1(+) CD49f(hi)/Trop-2(+) (LSC Trop-(2+)) surface phenotype was enriched in mesenchymal markers, especially EMT master regulator Slug, encoded by the Snail gene. To further dissect the role of Slug in mPSCs, we used transgenic Snai2(tm1.1wbg) reporter mouse strain. Using this model, we confirmed the presence of mesenchymal traits and increase of organoid forming capacity in Slug(+) population of mPSCs. The Slug(+)derived organoids comprised all prostate epithelial cell types basal, luminal, and neuroendocrine. Collectively, these data uncover the important role of Slug expression in the physiology of mouse prostate stem cells.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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