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G-quadruplexes in H1N1 influenza genomes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F21%3A00541909" target="_blank" >RIV/68081707:_____/21:00541909 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216305:26310/21:PU138597 RIV/00216224:14310/21:00121026 RIV/61988987:17310/21:A22029NU

  • Result on the web

    <a href="https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-07377-9" target="_blank" >https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-07377-9</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1186/s12864-021-07377-9" target="_blank" >10.1186/s12864-021-07377-9</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    G-quadruplexes in H1N1 influenza genomes

  • Original language description

    BackgroundInfluenza viruses are dangerous pathogens. Seventy-Seven genomes of recently emerged genotype 4 reassortant Eurasian avian-like H1N1 virus (G4-EA-H1N1) are currently available. We investigated the presence and variation of potential G-quadruplex forming sequences (PQS), which can serve as targets for antiviral treatment.ResultsPQS were identified in all 77 genomes. The total number of PQS in G4-EA-H1N1 genomes was 571. Interestingly, the number of PQS per genome in individual close relative viruses varied from 4 to 12. PQS were not randomly distributed in the 8 segments of the G4-EA-H1N1 genome, the highest frequency of PQS being found in the NP segment (1.39 per 1000nt), which is considered a potential target for antiviral therapy. In contrast, no PQS was found in the NS segment. Analyses of variability pointed the importance of some PQS, even if genome variation of influenza virus is extreme, the PQS with the highest G4Hunter score is the most conserved in all tested genomes. G-quadruplex formation in vitro was experimentally confirmed using spectroscopic methods.ConclusionsThe results presented here hint several G-quadruplex-forming sequences in G4-EA-H1N1 genomes, that could provide good therapeutic targets.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10603 - Genetics and heredity (medical genetics to be 3)

Result continuities

  • Project

    <a href="/en/project/EF15_003%2F0000477" target="_blank" >EF15_003/0000477: Structural gymnastics of nucleic acids: from molecular principles through biological functions to therapeutic targets.</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    BMC Genomics

  • ISSN

    1471-2164

  • e-ISSN

    1471-2164

  • Volume of the periodical

    22

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    11

  • Pages from-to

    77

  • UT code for WoS article

    000613379300001

  • EID of the result in the Scopus database

    2-s2.0-85099932787