Variability of fluorescence intensity distribution measured by flow cytometry is influenced by cell size and cell cycle progression

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F23%3A00574519" target="_blank" >RIV/68081707:_____/23:00574519 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/23:00132238 RIV/00216208:11130/23:10458314 RIV/00159816:_____/23:00079513 RIV/00064203:_____/23:10458314

Result on the web

<a href="https://www.nature.com/articles/s41598-023-31990-1" target="_blank" >https://www.nature.com/articles/s41598-023-31990-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-023-31990-1" target="_blank" >10.1038/s41598-023-31990-1</a>

Result language

angličtina

Original language name

Variability of fluorescence intensity distribution measured by flow cytometry is influenced by cell size and cell cycle progression

Original language description

The distribution of fluorescence signals measured with flow cytometry can be influenced by several factors, including qualitative and quantitative properties of the used fluorochromes, optical properties of the detection system, as well as the variability within the analyzed cell population itself. Most of the single cell samples prepared from in vitrocultures or clinical specimens contain a variable cell cycle component. Cell cycle, together with changes in the cell size, are two of the factors that alter the functional properties of analyzed cells and thus affect the interpretation of obtained results. Here, we describe the association between cell cycle status and cell size, and the variability in the distribution of fluorescence intensity as determined with flow cytometry, at population scale. We show that variability in the distribution of background and specific fluorescence signals is related to the cell cycle state of the selected population, with the 10% low fluorescence signal fraction enriched mainly in cells in their G0/G1 cell cycle phase, and the 10% high fraction containing cells mostly in the G2/M phase. Therefore we advise using caution and additional experimental validation when comparing populations defined by fractions at both ends of fluorescence signal distribution to avoid biases caused by the effect of cell cycle and cell size.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30102 - Immunology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Volume of the periodical

13

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

4889

UT code for WoS article

001017313600005

EID of the result in the Scopus database

2-s2.0-85150947560