Flow cytometric analysis of Saccharomyces cerevisiae

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F06%3A00017747" target="_blank" >RIV/60461373:22330/06:00017747 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Flow cytometric analysis of Saccharomyces cerevisiae
Original language description
Two methods of preservation and storage of yeast cultures are tested. Freest yeast population suspended at medium containing glycerol (5% v/v) as a cryoprotectant and periodic sub-culture using agar slopes (slants). This work was carried out with three widely used Czech lager strains - No. 2, 7 and 95. All yeasts were analyzed by flow cytometry prior to storage and after storage. Fluorescent intensity of DNA was analysed by flow cytometry and signal was used as a marker for cell cycle determination. During experiments were monitored changes in significant properties of yeast (glycogen, proteins, DNA) by fluorescent methods. It seems that the impact of storage conditions has not influence on basic characteristic of yeast population, dynamic of microbialgrowth, cell cycle and technological properties of yeast cultures.
Czech name
Analýza Saccharomyces cerevisiae průtokovou cytometrií
Czech description
V tomto příspěvku jsou porovnány dvě metody uchovávání laboratorních a průmyslových kmenů kvasinky Saccharomyces cerevisiae za použití průtokové cytometrie.Metody jsou zhodnoceny po stránce praktické a ekonomické náročnosti a dostupnosti.

Project
<a href="/en/project/1M0570" target="_blank" >1M0570: Research Centre for Study of Extract Compounds of Barley and Hop</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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