Fragmentation of nucleoli in senescent cancer cells is associated with increased levels of polyadenylated transcripts derived from noncoding regions of rDNA units
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F25%3A00644597" target="_blank" >RIV/68081707:_____/25:00644597 - isvavai.cz</a>
Result on the web
<a href="https://pmc.ncbi.nlm.nih.gov/articles/PMC12678568/" target="_blank" >https://pmc.ncbi.nlm.nih.gov/articles/PMC12678568/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00249-025-01773-9" target="_blank" >10.1007/s00249-025-01773-9</a>
Alternative languages
Result language
angličtina
Original language name
Fragmentation of nucleoli in senescent cancer cells is associated with increased levels of polyadenylated transcripts derived from noncoding regions of rDNA units
Original language description
In this study, we investigated the behavior of rDNA loci in senescent MCF-7 mammary cancer cells induced by gamma irradiation. To analyze changes in nucleolar structure we used rDNA-FISH and immunohistochemical staining with fibrillarin and UBF transcription factor. The expression levels of rDNAs and nucleolar proteins were determined by RNA-seq of total and poly-A libraries. The cytological and molecular parameters of nucleoli were monitored throughout the 7-day interval following irradiation. Senescent cells exhibited a higher proportion of smaller nucleoli as compared to cycling cells, indicating nucleolar fragmentation. The rDNA copy number and expression of rDNA variants remained stable in cycling and senescent cells. However, the levels of polyadenylated rRNA species derived from external (5 ' ETS) and internal (ITS1) rDNA spacers tend to increase (c.2 fold) following irradiation. At the protein level, senescent cells showed decreased levels of fibrillarin and UBF transcription factor while localization of both proteins in the nucleolus was not impaired. We conclude that withdrawal from cell cycle does not change expression patterns of rDNA variants. However, defects in rRNA processing may lead to fragmentation of nucleoli in senescent cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Biophysics Journal With Biophysics Letters
ISSN
0175-7571
e-ISSN
1432-1017
Volume of the periodical
54
Issue of the periodical within the volume
8
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
613-623
UT code for WoS article
001513459500001
EID of the result in the Scopus database
2-s2.0-105008822742