Chloride present in biological samples as a tool for enhancement of sensitivity in CZE analysis of trace analytes.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F01%3A25010054" target="_blank" >RIV/68081715:_____/01:25010054 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Chloride present in biological samples as a tool for enhancement of sensitivity in CZE analysis of trace analytes.
Original language description
Having acetoacetate, malate, citrate and some drug metabolites in untreated serum samples, the stacking mechanism of these compounds in an electrolyte system comprising 5 mM mandelic acid and e-aminocaproic acid (pH 3.8) is explained. Analytes are monitored by indirect UV absorption detection. Attention is paid to the minimum chloride concentration required with respect to the concentration ratio of phosphate (stacker) and lactate (destacker) present in the sample so as to ensure both stacking and separation of trace analytes. Insight into the separation process is given both with computer simulations and experiments. For selected analytes, the effect of the chloride concentration on quantitative evaluation, sensitivity and limit of detection is demonstrated as well.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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