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Analysis of ethyl glucuronide in human serum by capillary electrophoresis with sample self-stacking and indirect detection

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F05%3A00021363" target="_blank" >RIV/68081715:_____/05:00021363 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Analysis of ethyl glucuronide in human serum by capillary electrophoresis with sample self-stacking and indirect detection

  • Original language description

    Ethyl glucuronide (EtG), a metabolite of ethanol, is a marker of recent alcohol consumption. The capillary zone electrophoresis analysis of EtG in model mixtures and human serum is reported using uncoated and coated fused-silica capillaries together withacidic buffers in the pH range between 3.2 and 4.4 and indirect detection. In these approaches, separation of EtG from anionic serum components of high and low concentration is based upon transient isotachophoretic stacking. A buffer composed of 10 mM nicotinic acid and epsilon-aminocaproic acid (pH 4.3) is demonstrated to provide a detection limit for EtG in serum of 0.1 microg/ml, a value that is relevant for clinical and forensic purposes.

  • Czech name

    Analýza etylglukuronidu v lidském séru kapilární elektroforézou se self-stackingem vzorku a nepřímou detekcí

  • Czech description

    Ethyl glucuronid (EtG), metabolit ethanolu, je marker nedávné konzumace alkoholu. Analýza EtG kapilární zónovou elektroforézou v modelových směsích a lidském séru je popsána za použití nepokrytých a pokrytých křemených kapilár za použití kyselých pufrů vrozsahu pH mezi 3.2 a 4.4 a nepřímé detekce. V popsaných postupech je separace EtG od anionických složek séra vysoké a nízké koncentrace založen na efektech přechodné izotachoforézy. Pufr složený z 10 mM nikotinové kyseliny a epsilon-aminokapronové kyseliny (pH 4.3) umožnil dosáhnout detekční limit EtG 0.1 microg/ml, hodnotu, která je použitelná po klinické a soudní účely

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/IAA4031401" target="_blank" >IAA4031401: Development ov new optimizing and methodological techniques for capillary electrophoresis</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2005

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Chromatography A

  • ISSN

    0021-9673

  • e-ISSN

  • Volume of the periodical

    1081

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    7

  • Pages from-to

    2-8

  • UT code for WoS article

  • EID of the result in the Scopus database