Microfluidic device for concentration and SERS-based detection of bacteria in drinking water
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F21%3A00532403" target="_blank" >RIV/68081715:_____/21:00532403 - isvavai.cz</a>
Result on the web
<a href="http://hdl.handle.net/11104/0310907" target="_blank" >http://hdl.handle.net/11104/0310907</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.202000048" target="_blank" >10.1002/elps.202000048</a>
Alternative languages
Result language
angličtina
Original language name
Microfluidic device for concentration and SERS-based detection of bacteria in drinking water
Original language description
There is a constant need for the development of easy-to-operate systems for the rapid and unambiguous identification of bacterial pathogens in drinking water without the requirement for time-consuming culture processes. In this study, we present a disposable and low-cost lab-on-a-chip device utilizing a nanoporous membrane, which connects two stacked perpendicular microfluidic channels. Whereas one of the channels supplies the sample, the second one attracts it by potential-driven forces. Surface-enhanced Raman spectrometry (SERS) is employed as a reliable detection method for bacteria identification. To gain the effect of surface enhancement, silver nanoparticles were added to the sample. The pores of the membrane act as a filter trapping the bodies of microorganisms as well as clusters of nanoparticles creating suitable conditions for sensitive SERS detection. Therein, we focused on the construction and characterization of the device performance. To demonstrate the functionality of the microfluidic chip, we analyzed common pathogens (Escherichia coli DH5 alpha and Pseudomonas taiwanensis VLB120) from spiked tap water using the optimized experimental parameters. The obtained results confirmed our system to be promising for the construction of a disposable optical platform for reliable and rapid pathogen detection which couples their electrokinetic concentration on the integrated nanoporous membrane with SERS detection.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GJ20-14069Y" target="_blank" >GJ20-14069Y: Surface-enhanced Raman spectroscopy: Powerful tool for real time detection of electrophoretically separated phosphorylated biomolecules</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
1522-2683
Volume of the periodical
42
Issue of the periodical within the volume
1-2
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
86-94
UT code for WoS article
000535060400001
EID of the result in the Scopus database
2-s2.0-85085517630