SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F23%3A00570271" target="_blank" >RIV/68081731:_____/23:00570271 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/23:00078796 RIV/00216224:14110/23:00134625
Result on the web
<a href="https://www.mdpi.com/2079-6374/13/2/182" target="_blank" >https://www.mdpi.com/2079-6374/13/2/182</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/bios13020182" target="_blank" >10.3390/bios13020182</a>
Alternative languages
Result language
angličtina
Original language name
SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering
Original language description
Efficient separation and sensitive identification of pathogenic bacterial strains is essential for a prosperous modern society, with direct applications in medical diagnostics, drug discovery, biodefense, and food safety. We developed a fast and reliable method for antibody-based selective immobilization of bacteria from suspension onto a gold-plated glass surface, followed by detection using strain-specific antibodies linked to gold nanoparticles decorated with a reporter molecule. The reporter molecules are subsequently detected by surface-enhanced Raman spectroscopy (SERS). Such a multi-functionalized nanoparticle is called a SERS-tag. The presented procedure uses widely accessible and cheap materials for manufacturing and functionalization of the nanoparticles and the immobilization surfaces. Here, we exemplify the use of the produced SERS-tags for sensitive single-cell detection of opportunistic pathogen Escherichia coli, and we demonstrate the selectivity of our method using two other bacterial strains, Staphylococcus aureus and Serratia marcescens, as negative controls. We believe that the described approach has a potential to inspire the development of novel medical diagnostic tools for rapid identification of bacterial pathogens.
Czech name
—
Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/NU21-05-00341" target="_blank" >NU21-05-00341: Advanced methods in detection of pathogenic microorganisms and analysis the antimicrobials’ effect using Raman spectroscopy and other methods at the molecular level in optofluidic systems</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biosensors
ISSN
2079-6374
e-ISSN
2079-6374
Volume of the periodical
13
Issue of the periodical within the volume
2
Country of publishing house
CH - SWITZERLAND
Number of pages
14
Pages from-to
182
UT code for WoS article
000938961100001
EID of the result in the Scopus database
2-s2.0-85149147304