Sub-minute analysis of lactate from a single blood drop using capillary electrophoresis with contactless conductivity detection in monitoring of athlete performance
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F21%3A00546268" target="_blank" >RIV/68081715:_____/21:00546268 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/21:00124349
Result on the web
<a href="https://www.mdpi.com/1420-3049/26/19/5817" target="_blank" >https://www.mdpi.com/1420-3049/26/19/5817</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/molecules26195817" target="_blank" >10.3390/molecules26195817</a>
Alternative languages
Result language
angličtina
Original language name
Sub-minute analysis of lactate from a single blood drop using capillary electrophoresis with contactless conductivity detection in monitoring of athlete performance
Original language description
A simple and fast method for the analysis of lactate from a single drop of blood was developed. The finger-prick whole blood sample (10 µL) was diluted (1:20) with a 7% (w/v) solution of tris(hydroxymethyl)methylamino] propanesulfonic acid and applied to a blood plasma separation device. The device accommodates a membrane sandwich composed of an asymmetric polysulfone membrane and a supporting textile membrane that allows the collection of blood plasma into a narrow glass capillary in less than 20 s. Separated and simultaneously diluted blood plasma was directly injected into a capillary electrophoresis instrument with a contactless conductivity detector (CE-C4 D) and analyzed in less than one minute. A separation electrolyte consisted of 10 mmol/L L-histidine, 15 mmol/L DL-glutamic acid, and 30 µmol/L cetyltrimethylammonium bromide. The whole procedure starting from the finger-prick sampling until the CE-C4 D analysis was finished, took less than 5 min and was suitable for monitoring lactate increase in blood plasma during incremental cycling exercise. The observed lactate increase during the experiments measured by the developed CE-C4 D method correlated well with the results from a hand-held lactate analyzer (R = 0.9882). The advantage of the developed CE method is the speed, significant savings per analysis, and the possibility to analyze other compounds from blood plasma
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecules
ISSN
1420-3049
e-ISSN
1420-3049
Volume of the periodical
26
Issue of the periodical within the volume
19
Country of publishing house
CH - SWITZERLAND
Number of pages
9
Pages from-to
5817
UT code for WoS article
000717228700001
EID of the result in the Scopus database
2-s2.0-85115829560