Absolute Counting Method with Multiplexing Capability for Estimating the Number Concentration of Nanoparticles Using Anisotropically Collapsed Gels
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F22%3A00562009" target="_blank" >RIV/68081715:_____/22:00562009 - isvavai.cz</a>
Alternative codes found
RIV/68081723:_____/22:00562009
Result on the web
<a href="https://hdl.handle.net/11104/0334437" target="_blank" >https://hdl.handle.net/11104/0334437</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.2c02989" target="_blank" >10.1021/acs.analchem.2c02989</a>
Alternative languages
Result language
angličtina
Original language name
Absolute Counting Method with Multiplexing Capability for Estimating the Number Concentration of Nanoparticles Using Anisotropically Collapsed Gels
Original language description
The presented method is suitable for estimating the number concentration of nanoparticles and their bioconjugates. The method benefits from well-defined immobilization of nanomaterials in anisotropically collapsed agarose gel. Once immobilized, the nanoparticles are imaged by a microscope and counted. The number of counted nanoparticles is then used for estimating the number concentration. The accuracy and precision of the method were characterized by the samples of photon-upconversion nanoparticles. By analyzing the brightness of the emission from single diffraction-limited spots, it was possible to analyze also the content of UCNP clusters. The detection of extremely weak emitters was proved. The method supports several microscope imaging modalities such as photon-upconversion, fluorescence, dark-field, and bright-field microscopy. The applicability of these modalities is demonstrated by imaging UCNPs, dye-doped fluorescent silica nanoparticles, CdSe/ZnS quantum dots, and submicron silica particles. Thus, the method is not limited to a particular nanomaterial or imaging modality type. The method was developed for aqueous dispersions of nanoparticles and utilized the agarose gel to provide a well-defined immobilization of nanomaterials. However, the gels are not limited to only agarose or aqueous dispersions. Therefore, the method can be likely extended to other types of solvents and gel matrices. Interestingly, agarose contains only light elements such as carbon, oxygen, hydrogen, and a small amount of sulfur. Therefore, the method is potentially compatible with TEM, which can provide an even more detailed analysis.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA21-03156S" target="_blank" >GA21-03156S: Photon-upconversion labeling for microfluidic single-molecule immunoassays of protein biomarkers</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Chemistry
ISSN
0003-2700
e-ISSN
1520-6882
Volume of the periodical
94
Issue of the periodical within the volume
41
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
14340-14348
UT code for WoS article
000870033800001
EID of the result in the Scopus database
2-s2.0-85139477673