Preparativeseparationof immunoglobulins from bovine colostrum by continuous divergent-flow electrophoresis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F23%3A00562904" target="_blank" >RIV/68081715:_____/23:00562904 - isvavai.cz</a>
Result on the web
<a href="https://hdl.handle.net/11104/0335073" target="_blank" >https://hdl.handle.net/11104/0335073</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.202200679" target="_blank" >10.1002/jssc.202200679</a>
Alternative languages
Result language
angličtina
Original language name
Preparativeseparationof immunoglobulins from bovine colostrum by continuous divergent-flow electrophoresis
Original language description
Immunoglobulins in bovine colostrum were separated and fractionated from other proteins using method and instrumentation developed in our laboratory. The proposed separation was based on bidirectional isotachophoresis/moving boundary electrophoresis with electrofocusing of the analytes in pH gradient from 3.9 to 10.1. The preparative instrumentation included the trapezoidal non-woven fabric that served as separation space with divergent continuous flow. The defatted and casein precipitate free colostrum supernatant was loaded directly into instrument without any additional colostrum pre-preparation. Immunoglobulin G that was fractionated from other immune proteins such as bovine serum albumin, β-lactoglobulin and α-lactalbumin, was continuously collected in separated fractions over 3 hours. The fractions were further processed, and isolated immunoglobulin G in the liquid fractions was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and by re-focusing in gel isoelectric focusing. Separated immunoglobulin G was detected in seven fractions by sodium dodecyl sulphate-polyacrylamide gel electrophoresis with gradually decreased concentration in the fractions. Re-focusing of the proteins in the fractions by gel isoelectric focusing revealed multiple separated zones of immunoglobulin G with the pI values covering the range from 5.4 to 7.2. Each fraction contained the distinct zones with gradually increased pI values and decreased concentrations from fraction to fraction.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Separation Science
ISSN
1615-9306
e-ISSN
1615-9314
Volume of the periodical
46
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
2200679
UT code for WoS article
000876663100001
EID of the result in the Scopus database
2-s2.0-85141364876