Rhodamine B-based Labeling for Oligosaccharide and Glycan Analysis by CE/LIF

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F24%3A00587086" target="_blank" >RIV/68081715:_____/24:00587086 - isvavai.cz</a>
Result on the web
<a href="https://www.msb2024.org/user_uploads/program/Program_and_Abstract_Book_final.pdf" target="_blank" >https://www.msb2024.org/user_uploads/program/Program_and_Abstract_Book_final.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Rhodamine B-based Labeling for Oligosaccharide and Glycan Analysis by CE/LIF
Original language description
Fluorescent labeling represents a key step in oligosaccharide and glycan analysis by capillary electrophoresis with laser-induced fluorescence detection. In this work, we present a synthesis of the rhodamine B-based fluorescent tag modified by a hydrazide functionality for saccharide derivatization via hydrazone formation chemistry. The labeling conditions were optimized to obtain a high reaction yield. The labeled oligosaccharides and glycans were separated in polybrene-coated capillaries and the separation conditions (BGE compositions, separation, voltage, capillary length, etc.) were optimized. The labeled products were detected with a 532-nm laser and 550-nm longpass or 560/10-nm bandpass optical filters. The optimized labeling and separation conditions were applied for CE/LIF analysis of glycans released from model glycoproteins such as ribonuclease B and immunoglobulin G.
Czech name
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Czech description
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Project
<a href="/en/project/9F23002" target="_blank" >9F23002: Development of High Sensitivity and High Resolution Analytical Tools for the Brain Glycans</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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