Labeling of oligosaccharides and N-linked glycans by a rhodamine-based fluorescent tag for analysis by capillary electrophoresis with laser-induced fluorescence and mass spectrometry detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F25%3A00603181" target="_blank" >RIV/68081715:_____/25:00603181 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/25:00140682
Result on the web
<a href="https://hdl.handle.net/11104/0360419" target="_blank" >https://hdl.handle.net/11104/0360419</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.talanta.2024.127456" target="_blank" >10.1016/j.talanta.2024.127456</a>
Alternative languages
Result language
angličtina
Original language name
Labeling of oligosaccharides and N-linked glycans by a rhodamine-based fluorescent tag for analysis by capillary electrophoresis with laser-induced fluorescence and mass spectrometry detection
Original language description
In this work, we present the synthesis and application of fluorescent rhodamine B hydrazide for the derivatization of simple oligosaccharides and complex glycans using a hydrazone formation chemistry approach. The labeling conditions and the experimental setup of CE/LIF were optimized by analyzing oligosaccharide standards. The CE/LIF separations were performed in polybrene-coated capillaries eliminating the need for the purification step after derivatization. The addition of methanol to the background electrolyte significantly increased the LIF detection sensitivity reaching the limits of detection in the attomole range. The resolution of carbohydrate samples was improved by using long (98 cm) capillaries and polymer additives (polybrene). The developed method was applied for CE/LIF and CE-MS analysis of N-linked glycans released from bovine ribonuclease B and the therapeutic monoclonal antibody of trastuzumab.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Talanta
ISSN
0039-9140
e-ISSN
1873-3573
Volume of the periodical
286
Issue of the periodical within the volume
MAY
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
127456
UT code for WoS article
001412428700001
EID of the result in the Scopus database
2-s2.0-85213545134